This course is a review of the following;

Nature of metabolic reaction sequences, integration of metabolic pathways to emphasize the relation of TCA cycle to protein, carbohydrate, lipid and nucleic acids metabolism. Turnover rates and metabolic pools.  The basis of metabolic control; pacemakers/rate limiting enzymes. Mechanism of metabolic controls: (i) coarse metabolic control; induction and repression, catabolite repression -  lactose, tryptophan and arabinose operons (ii) fine metabolic control; including feedback inhibition, product inhibition, alteration of substrate or co-substrate concentration (substrate concentration, km values and binding affinity), variation in pH, allosteric regulation, covalent modification, subunit association/dissociation and reversible associations of multi-enzyme complexes. Identification of different regulatory mechanisms in metabolic pathways. Description of the mechanism of regulation of lipid, carbohydrate, and protein metabolism in the muscles and liver should be emphasised. Complexity of metabolism and concept of biochemical unity. The central role of Ca2+ in biochemical regulation Experimental approaches to metabolic pathways